Action Potential (AP), which is a transient change in membrane potential, can be generated by excitable cells. The AP firing plays central role in cell-to-cell communications.
In Acroscell, we offer the measurement of neuronal excitability on a single cell-level to evaluate the effects of pharmacological agents on distinct neuronal cell types, receptors, ion channels or neurotransmitter systems. Whole cell recordings are tailored to the specific characteristics and mechanisms of action of the investigational substance.
Fig.1 Physiological features of neurons particularly vulnerable in PD, SCAs and ALS, and how these features are affected in disease.
The goal of neuronal electrophysiology is to investigate how individual ionic currents and the interplay between currents in determining the profile and time course of AP. After passing neurotoxicity test, studies on drug discovery or drug safety should forward to how drugs affect neuronal electrophysiological functions like AP firing.
Fig.2 Analysis of AP initiation
Specifically, we can offer multi-parameter functional analysis including:
Action potential threshold
Action potential amplitude
Action potential depolarization time/repolarization time
Other action potential properties
Roselli F, Caroni P. From intrinsic firing properties to selective neuronal vulnerability in neurodegenerative diseases. Neuron. 2015; 85: 901–910.
Hedrich UBS, et al. Impaired Action Potential Initiation in GABAergic Interneurons Causes Hyperexcitable Networks in an Epileptic Mouse Model Carrying a Human Nav1.1 Mutation. J Neurosci. 2014; 34: 14874–14889.