Nav1.5 (SCN5A) is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. Nav1.5 is found primarily in cardiac muscle, where it mediates the fast influx of Na+ ions (INa) across the cell membrane, resulting in the fast depolarization phase of the cardiac action potential. As such, it plays a major role in impulse propagation through the heart. Hundreds of genetic variants have been found in patients with a long list of cardiac manifestations. Mutations in NaV1.5 induce a vast number of cardiac diseases:
Genetic variations in SCN5A are also linked to other inherited disorders characterized by structural alterations of the heart.
Fig. 1 Functional and qualitative representation of Dominant Negative variants L325R of Nav1.5
Fig. 2 Family of tracings of INa in NZs and IZ48h
Acroscell provides manual and auto patch clamp human Nav1.5 (hNav1.5) assay to evaluate drug's inhibition or potentiation effect on hNav1.5 channel. Pre-compound current and post-compound current are measured by patch clamp, and applied to the calculation of Nav1.5 inhibition.
Here at Acroscell, our protocols include screening study designs-single high concentration or IC50 screening- as well as GLP compliance monitoring and formulation analysis for Nav1.5.