For neuroscientists, the quest for truly predictive neural models has its challenges given the brain a very complex organ comprised of many diverse cell types, complex cell-to-cell interactions and intricate signaling pathways. The availability of well-characterized and immortalized neural cell lines has led to a rapid expansion of knowledge in many aspects of neurobiology. Unlike primary cells, the major advantages of cell lines are that they are capable of long-term or indefinite growth, and generally represent a single cell type, providing a degree of reproducibility and simplicity in an otherwise complicated biological field. With these advantages come disadvantages as well, but careful experimental design, and an appreciation of the limits of the approach, allows the investigator using neural cell lines a powerful tool in answering precise questions with a minimum of interpretational complications. Furthermore, the use of cell lines eliminates the vast cost and other concerns associated with animals as a source of experimental material.
Acroscell specializes in providing the highest quality neuronal cell lines with strict quality control. We also supply clients with media supplements, adhesion substrates and neuro-modulatory compounds for neuronal cell culture research. All of our reagents for culturing cells of the nervous system are designed to help maintain consistency within and across your experiments. Since neuronal cell lines also play an important role in the analysis of problems in electrophysiological and synaptic functions, we offer clients a comprehensive analysis of compound effects on neuronal functions.
The capability of long-term growth and ease to cultivate make the use of immortalized cell lines important tools in neuroscience studies. Additionally, when neural stem cells (NSCs) are used, they have an added benefit of self-renewal and differentiation along specific pathways to generate physiological relevant neuronal cell types of the central nervous system (CNS).
Consistent and dependable neural stem cell culture
Immortalized to produce a robust and consistent NSC culture
Offers phenotype and genotype stability
Includes multipotential neuronal differentiation capacity
Can be cultured in optimized defined serum-free media
Here are some example cells we provide:
Neuro 2A (N2A) Mouse Albino Neuroblastoma
N2A is a mouse neural crest-derived cell line that has been extensively used to study neuronal differentiation, axonal growth and signaling pathways.
ND7/23 Mouse Neuroblastoma X Rat Neuron
The ND7/23 immortalized rat dorsal root ganglion (DRG)-mouse N18Tg2 neuroblastoma hybrid cell line has shown great utility for transient and stable expression of exogenous ion channels and receptors in a native milieu, yielding useful fluorescence and electrophysiology assays for academic and drug discovery applications.
The SH-SY5Y cell line has become a popular cell model for PD research because this cell line possesses many characteristics of DAergic neurons. For example, these cells express tyrosine hydroxylase and dopamine-β-hydroxylase, as well as the dopamine transporter. Moreover, this cell line can be differentiated into a functionally mature neuronal phenotype in the presence of various agents. Upon differentiation, SH-SY5Y cells stop proliferating and a constant cell number is subsequently maintained.
As one of the most intensively studied cell lines in neurobiology, PC12 cell line was originally derived from a rat pheochromocytoma. PC12 cells can differentiate to a sympathetic neuron-like fate in response to nerve growth factor (NGF). PC12 cells are an excellent model system to analyze the molecular mechanisms underlying neuronal differentiation and NGF response. PC12 cells were one of the first cell lines used to study the mechanisms regulating changes in gene expression in response to growth factors.
Fig.1. Cardiac fibroblasts promote PC12 cells axonal/neuritogenesis through NGF paracrine action.
Human iPSC Derived Neural Progenitors
Blood Brain Barrier hCMEC/D3 Cell Line
Human Oligodendrocyte Differentiation Kit
ReNcell CX Human Neural Progenitor Cell Line
ReNcell VM Human Neural Progenitor Cell Line
N27 Rat Dopaminergic Neural Cell Line
Adult Rat Hippocampal
HAPI Rat Microglial Cell Line
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
Mouse Cranial Neural Crest Cell Line O9-1
Mouse Cortical NSC
Mouse Spinal Cord NSC
Cell lines of spontaneous origin or derived from tumors are relatively few. Chemical transformation and somatic cell hybridization service in Acroscell have shown that cell lines can be obtained from particular cell populations. We provide efficient techniques, genetically defined way to immortalize specifically targeted cell populations. We provide Genetically Engineered Cell Lines using our CRISPR/Cas9 gene-editing platform for customized cell line development which is optimized for mammalian and human cells.
Rigorous procedure in Acroscell helps quickly evaluate the feasibility of using proper cell line. Our genetic modifications of your chosen in-house cell line optimize the use of your existing data and avoid the need for new standardization experiments.
Acroscell ensures reliable and reproducible performance in constructing stable murine- or human-derived cell lines.
Lendahl U and McKay RD. The use of cell lines in neurobiology. Trends Neurosci. 1990; 13: 132-137.
Murayama, Kaoru, et al. "Neural cell lines." Protocols for neural cell culture. Humana Press, 2001: 219-228.
Mias C, et al. Cardiac fibroblasts regulate sympathetic nerve sprouting and neurocardiac synapse stability. PLoS One. 2013; 8: e79068.