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Cultured Neuronal Cell Lines

For neuroscientists, the quest for truly predictive neural models has its challenges given the brain a very complex organ comprised of many diverse cell types, complex cell-to-cell interactions and intricate signaling pathways. The availability of well-characterized and immortalized neural cell lines has led to a rapid expansion of knowledge in many aspects of neurobiology. Unlike primary cells, the major advantages of cell lines are that they are capable of long-term or indefinite growth, and generally represent a single cell type, providing a degree of reproducibility and simplicity in an otherwise complicated biological field. With these advantages come disadvantages as well, but careful experimental design, and an appreciation of the limits of the approach, allows the investigator using neural cell lines a powerful tool in answering precise questions with a minimum of interpretational complications. Furthermore, the use of cell lines eliminates the vast cost and other concerns associated with animals as a source of experimental material.

Creative Bioarray specializes in providing the highest quality neuronal cell lines with strict quality control. We also supply clients with media supplements, adhesion substrates and neuro-modulatory compounds for neuronal cell culture research. All of our reagents for culturing cells of the nervous system are designed to help maintain consistency within and across your experiments. Since neuronal cell lines also play an important role in the analysis of problems in electrophysiological and synaptic functions, we offer clients a comprehensive analysis of compound effects on neuronal functions.

Advantages

The capability of long-term growth and ease to cultivate make the use of immortalized cell lines important tools in neuroscience studies. Additionally, when neural stem cells (NSCs) are used, they have an added benefit of self-renewal and differentiation along specific pathways to generate physiological relevant neuronal cell types of the central nervous system (CNS).

  • Consistent and dependable neural stem cell culture

  • Immortalized to produce a robust and consistent NSC culture

  • Offers phenotype and genotype stability

  • Includes multipotential neuronal differentiation capacity

  • Can be cultured in optimized defined serum-free media

Categories

Here are some example cells we provide:

  • Neuro 2A (N2A) Mouse Albino Neuroblastoma

N2A is a mouse neural crest-derived cell line that has been extensively used to study neuronal differentiation, axonal growth and signaling pathways.

  • ND7/23 Mouse Neuroblastoma X Rat Neuron

The ND7/23 immortalized rat dorsal root ganglion (DRG)-mouse N18Tg2 neuroblastoma hybrid cell line has shown great utility for transient and stable expression of exogenous ion channels and receptors in a native milieu, yielding useful fluorescence and electrophysiology assays for academic and drug discovery applications.

  • SH-SY5Y cells, Human Neuroblastoma

The SH-SY5Y cell line has become a popular cell model for PD research because this cell line possesses many characteristics of DAergic neurons. For example, these cells express tyrosine hydroxylase and dopamine-β-hydroxylase, as well as the dopamine transporter. Moreover, this cell line can be differentiated into a functionally mature neuronal phenotype in the presence of various agents. Upon differentiation, SH-SY5Y cells stop proliferating and a constant cell number is subsequently maintained.

  • PC12 cell line

As one of the most intensively studied cell lines in neurobiology, PC12 cell line was originally derived from a rat pheochromocytoma. PC12 cells can differentiate to a sympathetic neuron-like fate in response to nerve growth factor (NGF). PC12 cells are an excellent model system to analyze the molecular mechanisms underlying neuronal differentiation and NGF response. PC12 cells were one of the first cell lines used to study the mechanisms regulating changes in gene expression in response to growth factors.


4-1-10 Cultured Neuronal Cell Lines-1.jpg

Fig.1. Cardiac fibroblasts promote PC12 cells axonal/neuritogenesis through NGF paracrine action.


Human-derived cells

  • Human iPSC Derived Neural Progenitors

  • Blood Brain Barrier hCMEC/D3 Cell Line

  • Human Oligodendrocyte Differentiation Kit

  • ReNcell CX Human Neural Progenitor Cell Line

  • ReNcell VM Human Neural Progenitor Cell Line

Rat-derived cells

  • N27 Rat Dopaminergic Neural Cell Line

  • Adult Rat Hippocampal

  • HAPI Rat Microglial Cell Line

Mouse-derived cells

  • GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line

  • Mouse Cranial Neural Crest Cell Line O9-1

  • Mouse Cortical NSC

  • Mouse Spinal Cord NSC

Customized neuronal cell lines

Cell lines of spontaneous origin or derived from tumors are relatively few. Chemical transformation and somatic cell hybridization service in Creative Bioarray have shown that cell lines can be obtained from particular cell populations. We provide efficient techniques, genetically defined way to immortalize specifically targeted cell populations. We provide Genetically Engineered Cell Lines using our CRISPR/Cas9 gene-editing platform for customized cell line development which is optimized for mammalian and human cells.

Rigorous procedure in Creative Bioarray helps quickly evaluate the feasibility of using proper cell line. Our genetic modifications of your chosen in-house cell line optimize the use of your existing data and avoid the need for new standardization experiments.

Creative Bioarray ensures reliable and reproducible performance in constructing stable murine- or human-derived cell lines.

References

  1. Lendahl U and McKay RD. The use of cell lines in neurobiology. Trends Neurosci. 1990; 13: 132-137.

  2. Murayama, Kaoru, et al. "Neural cell lines." Protocols for neural cell culture. Humana Press, 2001: 219-228.

  3. Mias C, et al. Cardiac fibroblasts regulate sympathetic nerve sprouting and neurocardiac synapse stability. PLoS One. 2013; 8: e79068.


For research use only.

Related Section

  • Xenopus Oocyte Screening Model
  • Acute Isolated Cardiomyocytes
  • Acute Dissociated Neurons
  • Primary Cultured Neurons
  • iPSC-derived Cardiomyocytes/Neurons
  • Acute/Cultured Organotypic Brain Slices
  • Oxygen Glucose Deprivation Model
  • 3D Cell Culture
  • iPSC-derived Neurons
  • Isolation and culture of neural stem/progenitor cells

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