Acroscell is experienced in epilepsy research with keeping many mouse models of epilepsy caused by of ion channel genes mutations. Both multiple genes mutations and single-gene perturbations induced epilepsy models are available in Acroscell. Our large variety of mouse models can help researchers in understanding epilepsy and drug therapy in epilepsy disorders. These models also help us to discover the genes that cause epilepsy in human patients as well as those genes that have an effect on our susceptibility to seizures.
Epilepsy disorders, also known as seizure disorders, characterized by chronically recurring seizures without clear precipitants. The term epilepsy refers to a spectrum of brain disorders resulting from a disturbance of the normal pattern of neuron activity. As a group of disorders, it is one of the most common diseases of the nervous system: the age-adjusted prevalence of epilepsy is in the range of 4 to 10 per 1000 people in most locations. While its diverse manifestations and causes demonstrate the complex nature of accurately diagnosing and treating any one form of epilepsy.
Epilepsy brings great burden to both the individuals with epilepsy and society. Because the diverse causes of epileptic disorders, the effectiveness of the available therapies (including drug therapy, diet therapy and nerve stimulation) varies significantly from patient to patient. In order to develop new medications, it is necessary to utilize experimental animal models of epilepsy. For example, chemically-induced kindling with pentylenetetrazole (PTZ) or electrically-induced seizures can be used a model of epilepsy. Identifying mouse models that recapitulate human epilepsies helps researchers in understanding of anticonvulsant drugs.
Fig. 1 EEG recording of a spike-and-wave discharge in GAERS in different brain areas (SoCx first two traces) and Motor cortex (last two traces)
In human epilepsy disorders, essential tremors and spontaneous epileptic seizures are disorders common in humans. Whether it's using in vitro or in vivo electrophysiological approaches to investigate mechanism, whole animal behavior or molecular biology techniques to better understand function or side effect profiles, our research teams will provide expert consultancy in putting together the study proposal you require, and our investigators will then rapidly deliver data of the highest quality to drive forward your research programs.
Fig.2 Nav1.6+/− mice kindle more slowly than wildtype littermates.
Acroscell has considerable expertise in a number of other key areas of neuroscience, including particular experience in providing firs-class preclinical translational research into epilepsy. Our tests for epilepsy animal models include:
In vivo: single unit recordings from most characterized brain regions; local field potential/population spike recording from hippocampus/cortex
In vitro: whole-cell patch-clamp recordings from brain slices (current/voltage/blind/visualized); local field potentials - spontaneous and evoked extracellular recordings from brain slices
Ion channel pharmacology: state-dependency, kinetics, and site of action using cultured and primary dissociated neurons, cell lines
Acroscells also put efforts in developing precisely tailored research packages to target out client's needs.
Giblin KA, Blumenfeld H. Is epilepsy a preventable disorder? New evidence from animal models. Neuroscientist. 2010; 16: 253–275.