Creative Bioarray
  • USA:
  • Europe:
LOGO
  • Home
    • About Us
    • Contact Us
    • Events
  • Screening
    • Ionic Screening Service
      • Ion Flux Assay
      • Fluorescence Assay
      • Patch-clamp Assay
    • Ionic Screening Panel
      • Sodium Channels
      • Potassium Channels
      • Chloride Channels
      • Calcium Channels
      • TRP Channels
      • ATP gated P2X Channels
      • ASICs
      • Nicotinic Acetylcholine Receptors
      • Ionotropic Glutamate-gated Receptors
      • GABAa Receptors
      • Glycine Receptors
      • 5-HT Receptors3
      • Cystic Fibrosis Transmembrane Conductance Regulators (CFTR)
      • Other Ion Channels
    • Stable Cell Lines
      • Ion Channel Expressing Cell
      • GPCR Expressing Cell
      • Other Membrane Receptors/Transporters/Exchangers Expression Cell
      • Customized Ionic Channel Expression System
  • Cardiology
    • Cardiac Ion Channel Screen Panels
      • HERG (IKR, KV11.1)
      • Cav1.2
      • Kv4.3
      • KCNQ1/mink/Kv7.1
      • Kir2.1
      • Nav1.5
      • Kv1.5
      • HCN1&HCN4
      • Other Ion Channels
    • CiPA
      • CiPA Cardiac Channel Assays
      • CiPA In silico Modeling
      • CiPA Translational Assays
    • 3D Cardiotoxicity
    • Cardiomyocyte Functions
      • Action Potential Properties
      • Calcium Transient in Cardiomyocytes
      • QT Service Using Langendorff Perfused Heart
    • Cardiac in vivo Assays
      • QRS, QT Waves Detection by ECG
  • Neurology
    • Neurotransmission Functions
      • sEPSC/sIPSC Recordings
      • fEPSP / Populational Spikes Recordings
      • LTP & LTD Formation or Deficit Detection
    • Basic Neuronal Activity
      • Action Potential Properties Assay
      • NMDA/AMPA Ratio Test
      • Calcium Signaling Test
      • Customized Electrophysiological Test
    • Neuronal in vivo Assays
      • In vivo Extra-cellular Recordings/Whole-cell Patch-clamp Recordings
      • Continuous Telemetric EEG Recordings
    • Animal Behavior Tests
      • Spontaneous Locomotor Functions
      • Cognitive Functions
      • Anxiety-Depression and Social Interaction Evaluation
      • Assays for Neuropsychiatric Disorders
  • Ophthalmology
  • Platform
    • Experiment Systems
      • Xenopus Oocyte Screening Model
      • Acute Isolated Cardiomyocytes
      • Acute Dissociated Neurons
      • Primary Cultured Neurons
      • Cultured Neuronal Cell Lines
      • iPSC-derived Cardiomyocytes/Neurons
      • Acute/Cultured Organotypic Brain Slices
      • Oxygen Glucose Deprivation Model
      • 3D Cell Culture
      • iPSC-derived Neurons
      • Isolation and culture of neural stem/progenitor cells
    • Animal Models
      • Alzheimer's Disease Model
      • Parkinson's Disease Model
      • Huntington's Disease Model
      • Epilepsy Model
      • ALS Model
      • Psychiatric Model
      • Autism Spectrum Disorder
      • Cerebral-Spinal Injury Model
      • Pain Model
      • Stroke Model
      • Fragile X Model
      • Acute/Chronic Heart Failure Model
    • Techinques
      • Manual Patch-clamp Technique
      • Automated Patch-clamp
      • Multi-Electrode Array (MEA)
      • FluxOR™ Thallium Assay
      • FLIPR Detection System
      • Optogenetics
      • Neuronal Tract Tracing
    • Resource
      • Technical Methods
      • Neuroscience
      • Channelopathies
      • Channelomics
    • Equipment
  • Order
  • Careers
  • Home
  • Platform
  • Experiment Systems
  • Acute Isolated Cardiomyocytes
  • Screening
  • Ionic Screening Service Ionic Screening Panel Stable Cell Lines
  • Cardiology
  • CiPA 3D Cardiotoxicity Cardiomyocyte Functions Cardiac in vivo Assays Cardiac Ion Channel Screen Panels
  • Neurology
  • Basic Neuronal Activity Neurotransmission Functions Neuronal in vivo Assays Animal Behavior Tests
  • Platform
  • Experiment Systems Animal Models Techinques Resource Equipment

Acute Isolated/Cultured Cardiomyocytes

Isolated adult cardiac myocytes maintained in primary culture have been used as a model of the adult myocardium for more than 30 years. With the recent advances and current increasing interests in using molecular biological techniques to investigate cardiac physiology, culturing myocytes is becoming an increasingly important technique.

To study and understand the mechanisms underlying normal cardiovascular function, cardio-protection, and cardiovascular diseases, Creative Bioarray uses cultured primary cardiomyocyte which can be easily isolated and cultured displaying highly viable and functional properties for researches.


4-1-3 Acute Isolated Cultured Cardiomyocytes-1.jpg

Fig. 1 Immunofluorescence images of z-disk organization in adult cardiomyocytes after 48-h


Advantages

Compared with whole heart, cardiomyocyte cultures are relatively pure, with limited contaminating cell types such as endothelial cells. Thus, cardiomyocyte cultures provide a homogeneous population of single cells, which are easy to visualize and manipulate. In addition, preparations of heart cells isolated from small mammals like mouse and rat enable a large number of quick, relatively low-cost experiments compared to studies conducted in whole animals.

Usage of isolated cardiac myocytes has a number of additional advantages such as the ability to select cells from different areas of the heart including the atria, left/right ventricle, the conductive system or a specific region of the heart following myocardial infarction. As well, while imaging techniques are often limited in thick tissue, isolated cells are well-suited for experiments aimed at visualizing cellular structure and the precise localization of intracellular molecules. Isolated cardiomyocytes are also routinely used for studies examining intracellular Ca2+ homeostasis, cellular mechanics, protein biochemistry and can be easily infected or transfected for gene transfer studies. Thus, isolated cardiomyocytes can be utilized in an array of experimental settings to provide valuable insight into cellular and sub-cellular physiology. By combining these data with experimental findings made in vivo and in intact tissue, animal models can be employed to examine cardiac function in a hierarchical manner.

While immortalized cardiac cell lines such as HL-1 cells are commercially available, acutely isolated cells are more physiologically relevant both structurally and functionally to the living organism. Cardiomyocyte isolation techniques are therefore of critical importance, as are methods for cell culture which aim to preserve as closely as possible the in vivo integrity and function of the cardiomyocytes.


4-1-3 Acute Isolated Cultured Cardiomyocytes-2.jpg

Fig. 2 Typical alterations in cardiomyocyte morphology during culturing


During cardiomyocyte isolation, since the heart is a solid organ with strong intercellular attachments, which makes the dissociation process more difficult and time consuming. Thus, isolating primary cardiomyocytes from neonatal mouse or rat hearts has typically been a time-consuming, labor-intensive task. Isolating primary cardiomyocytes is a delicate process involving the controlled use of enzymes to disrupt complex protein and intercellular matrix interactions found in heart tissue. Creative Bioarray has screened several different proteases and collagenases individually and in combination to determine the most efficient digestion strategy.

The Action Potential Kinetics (APD) aka Purkinje Repolarisation assay can be conducted in cardiac Purkinke fibers, ventricular strips or ventricular papillary muscles. The shape of the action potentials and the complement of ionic currents which underline the action potentials is different in the various tissues, and proper selection of the tissues exposed ensures the best sensitivity for the assay. Creative Bioarray isolates cardiac Purkinke fibers, ventricular strips or ventricular papillary muscles according to customers' requirements.

Isolated cardiomyocytes from small rodents are an invaluable tool in the study of cellular level function and regulation of electrophysiology, intracellular calcium fluxes, contractile mechanics and protein expression. The ability to culture myocytes, even for short times, further benefits researchers by permitting the incorporation of transgenes into cells and facilitating longer-term treatments. Combined with the recent availability of transgenic animals, the array of techniques for manipulating cellular physiology in the investigation of molecular mechanisms regulating function has vastly expanded.

Although myocytes have been isolated and cultured for a number of years by standard techniques, it remains somewhat of an art to consistently produce high-quality cells. Creative Bioarray has knowledge of the described reagents and protocols, along with common caveats and critical points in the procedures will enable our customers to more easily establish cell-based research strategies, and advance the pursuit of therapeutics for human disease.

References

  1. Loucha WE, et al. Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer. J Mol Cell Cardiol. 2011; 51: 288–298.

  2. Galie PA, et al. Substrate stiffness affects sarcomere and costamere structure and electrophysiological function of isolated adult cardiomyocytes. Cardiovasc Pathol. 2013; 22: 219–227.


For research use only.

Related Section

  • Xenopus Oocyte Screening Model
  • Acute Dissociated Neurons
  • Primary Cultured Neurons
  • Cultured Neuronal Cell Lines
  • iPSC-derived Cardiomyocytes/Neurons
  • Acute/Cultured Organotypic Brain Slices
  • Oxygen Glucose Deprivation Model
  • 3D Cell Culture
  • iPSC-derived Neurons
  • Isolation and culture of neural stem/progenitor cells

Inquiry


Subscribe
  • Links
  • Sodium Channels
  • Calcium Channels
  • Potassium Channels
  • Chloride Channels
  • TRP Channels
  • ATP gated P2X Channels
  • ASICs
  • USA
  • Europe

Copyright © 2025 Creative Bioarray. All rights reserved.